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KMID : 0984720090410030181
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Infection and Chemotherapy
2009 Volume.41 No. 3 p.181 ~ p.184
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Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method
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Kim Jun-Young
Kim Seong-Han
Lee Bok-Kwon
Jeon Se-Mi
Rhie Ho-Geun
Park Mi-Sun
Lee Hoan-Jong
Lee Ji-na
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Abstract
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A multiplex PCR method has been developed to classify extended spectrum β-lactamase (ESBL) and plasmid-mediated AmpC β-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type β-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL-producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of β-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.
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KEYWORD
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Extended Spectrum-β Lactamase (ESBL), Rapid classification, Multiplex PCR
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